Contents

Editors......................................................................................................................vii

List of abbreviations.................................................................................................ix

Chapter 1

Bioprocessing of viral vaccines–Introduction ................................1

Amine Kamen and Laura Cervera

Chapter 2

Introduction to virology..................................................................17

Shantoshini Dash

Chapter 3

Introduction to basic immunology and vaccine design ..............35

Alaka Mullick and Shantoshini Dash

Chapter 4

Cell lines for vaccine production...................................................57

Isabelle Knott, Jean-Philippe Matheise, Isabelle Ernest, and

Jean-Pol Cassart

Chapter 5

Upstream processing for viral vaccines–General aspects...........79

Lars Pelz, Sven Göbel, Karim Jaen, Udo Reichl, and Yvonne

Genzel

Chapter 6

Upstream processing for viral vaccines-Process

intensification .................................................................................137

Sven Göbel, Lars Pelz, Udo Reichl, and Yvonne Genzel

Chapter 7

Downstream processing of viral-based vaccines........................175

Rita P. Fernandes, Cristina Peixoto, and Piergiuseppe Nestola

Chapter 8

Analytics and virus production processes..................................201

Emma Petiot

Chapter 9

Manufacturing of seasonal and pandemic influenza

vaccines–A case study ...................................................................225

Cristina A. T. Silva, Shantoshini Dash, and Amine Kamen

v